FIG 3.

Genetic complementation of the mutations in the essential com genes of the selected nontransformable isolates. Genetic complementation was performed in the nontransformable isolates or corresponding mutants of R6 with the pIB166 carrying the wild-type comGC (A), coiA (B), and comE (C, D, and E). The transformants are presented as the mean CFU ± the standard errors from triplicate samples. R6 mutants with a deletion in each of the corresponding com genes and their complementation counterparts were included as controls.