FIG 2.

Interaction of FbaA and GAPDH with extracellular matrix proteins. (A) SDS-PAGE of the StrepTag-purified FbaA and GAPDH. (B) SDS-PAGE of plasminogen, vitronectin, fibrinogen, fibronectin, and BSA as a control. (C) Far-Western blot analyses of matrix proteins using purified GAPDH and FbaA as ligands. (D) Summary of the interactions between the matrix proteins and GAPDH and FbaA. The degree of interaction is indicated as follows: +++, strong; ++, intermediate; +, weak; − none. The theoretical masses of FbaA (30.8) and GAPDH (36.2 kDa) were lower than the estimated sizes in SDS-PAGE gels, which were 36 and 42 kDa, respectively. Arrows indicate the alpha, beta, and gamma chains of fibrinogen.