FIG 5.

FbaA and GAPDH interact with subfragments of the major amidase (Atl). (A) Far-Western blotting of GAPDH and FbaA with SA113 Δspa ΔsrtA and SA113 ΔatlA cells. (B) Binding of mCherry and GAPDH-mCherry to JE2 as well as to its fnbA, fnbB, and atl transposon mutants; binding was quantified by measurement of relative fluorescence units (****, P < 0.0001, by t test). (C) Far-Western blot analyses of recombinant Atl fragments (AM-R1/2, R1/2, AM [amidase] and GL [glucosaminidase]) purified from E. coli. GAPDH and FbaA were used as ligands; for detection, specific anti-FbaA and anti-GAPDH antibodies were used. As a control, no bait protein was added.