FIG 5.

Intradermal immunization with PspA provides protection against septicemia. Mice were immunized with 5.0 μg of PspA plus 1.0 μg of LT-IIb(T13I) (vaccinated) or with PBS (sham). Survival (A) and bacterial burden in the bloodstream (B) were measured for mice injected i.p. with EF3030 bacteria dispersed from biofilms in response to heat. (C) Levels of inflammatory cytokines in the blood at the time of euthanasia up to 24 h following bacterial challenge. Each dot represents an individual mouse (n = 5). Statistical analysis was performed using a log rank test for survival data in panel A or a Mann-Whitney U test for bacterial burden and cytokine data (panels B and C). *, P < 0.05; **, P < 0.01; ***, P < 0.001. Panels A and B include data from two independent experiments, whereas panel C includes data from one of the experiments included in the other panels.