FIG 1.

B. abortus and its LPS do not induce the cell death of mouse PMNs. (A) Heparinized blood of humans or C57BL/6 mice was incubated with B. abortus 2308-GFP at the indicated MOI for 2 h, and the PMN population was gated and analyzed by cytometry using annexin V as a cell death marker. (B) Heparinized blood of C57BL/6 mice was incubated with B. abortus 2308-GFP at an MOI of 10 (green line) or an MOI 100 (blue line) or with PBS (red line) for 2 h, and the PMN population was gated and analyzed by cytometry using annexin V as a cell death marker. (C) Heparinized blood of humans or C57BL/6 mice was incubated with Br-LPS at the indicated concentrations for 2 h, and the PMN population was gated and analyzed by cytometry for cell death markers using AquaDead and annexin V. The proportions of PMNs positive for the respective marker are presented with each section of the corresponding cytogram. While human PMNs acquire death cell markers, mouse PMNs remain unaffected. The figure represents one experiment of at least four repetitions.