FIG 7.

Induction of mouse PMN cell death after phagocytosis of antibody-opsonized smooth B. abortus 2308 or the rough Δper mutant. (A) Heparinized blood of C57BL/6 mice was incubated with B. abortus 2308-GFP or with B. abortus 2308-GFP opsonized with antibody (Ab) against Br-LPS at the indicated MOIs for 2 h, and then the PMN population was gated and analyzed by cytometry using annexin V as a cell death marker. Negative controls were treated with either PBS or antibody alone. Similar results were observed with bone marrow-derived PMNs. (B) Bone marrow PMNs of BALB/c mice were incubated with the rough mutant B. abortus Δper at an MOI of 50 or with PBS for 4 h, and the PMN population was gated and analyzed by cytometry using annexin V as a cell death marker. The figure represents one experiment of at least four repetitions.