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Replication of C. burnetii in bovine MDM (A) and in alveolar macrophages (B). Cells were inoculated with C. burnetii strains NMI or NMII (MOI of 100 for 1 h), and C. burnetii-specific genome equivalents (GE) were quantified in duplicate at different time points by icd-specific real-time PCR and calculated by use of a standard curve. Kinetic of C. burnetii replication was monitored in 3 independent experiments depicted by different symbols within the curves.
