FIG 4.
Expression of SrbA target genes in the AkuBKU80, ΔrbdA, and ΔsrbA strains. Northern blot analysis of the mRNA levels of SrbA target genes is shown for the AkuBKU80 wild-type and ΔrbdA and ΔsrbA mutant strains before (0 h) and after (3 h) a shift to a hypoxic atmosphere. All strains were initially cultivated for 16 h in MM (106 spores/ml inoculum) under normoxic conditions and then shifted for 3 h to hypoxic conditions (0.2% O2). Ten micrograms of RNA was loaded per lane, and rRNA bands are shown as a control for equal loading.