FIG 1.

Upregulation of IL-32, IL-6, and IL-8 expression in E. coli-infected cells. BME-UV or PS cells (2 × 10⁵) were grown in six-well plates for 24 h. The cells were then exposed to the E. coli K-12 strain (MOI, 30:1) for 8 h, 24 h, and 48 h. Isolation of total RNA, synthesis of cDNA, and qRT-PCR were performed as described in Materials and Methods. After normalization using PPIA and RPL19 genes, interleukin expression at 8 h, 24 h, and 48 h p.i. was calculated relative to the values obtained from mock cells, arbitrarily set to 1. Data were calculated from three different experiments performed in triplicate. P values of <0.05 (*) and <0.01 (**) were considered significant.