TABLE 1.
Primer sequences for qRT-PCRa
| Primer | Sequence | Product size (bp) | NCBI accession no. |
|---|---|---|---|
| IL-32f | 5′-TCAAGAGAACAGTCCCGAAACC-3′ | 71 | Consensus sequence |
| IL-32r | 5′-AGCGTACTTCTTGCTGTGCTTC-3′ | ||
| IL-8f | 5′-TGGGCCACACTGTGAAAAT-3′ | 92 | NM_173925.2 |
| bIL-8r | 5′-TCATGGATCTTGCTTCTCAGC-3′ | ||
| IL-6f | 5′-TGCTGGTCTTCTGGAGTATC-3′ | 153 | EU276071 |
| IL-6r | 5′- GTGGCTGGAGTGGTTATTAG-3′ | ||
| RPL19f | 5′-TACTGCCAATGCTCGAATGC-3′ | 114 | NM_001040516.1 |
| RPL19r | 5′-TGATACATGTGGCGGTCAATC-3′ | ||
| PPIAf | 5′-ATGGCAAGACCAGCAAGAAG-3′ | 201 | XM_002690515.2 |
| PPIAr | 5′-CTTGGAGGGGGATAAGGAAA-3′ |
a
Primer sequences were designed using primer 3. The RPL19 and PPIA genes were used as housekeeping genes. All melting temperatures were 60°C. The relative quantification of the mRNA levels of the target genes was determined using CFX Manager based on the threshold cycle method.