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CheD specifically interacts with MCP3 and MCP4. Affinity blotting was performed as described in the text. Purified Strep-CheD was probed with MBP-MCP3, MBP-MCP4, or MBP-MCP5, followed by immunoblotting with monoclonal MBP antibodies (Thermo Scientific). The bands at an apparent molecular mass of 19 kDa (Strep-CheD; arrows) were always observed in blots probed with MCP3 and MCP4, but not MCP5.
