TABLE 3.
Sample | CAS assay | Inhibition zone (mm) |
---|---|---|
Raw extract | Yellow | 20 |
F1 | Blue | 0 |
F2 | Blue | 0 |
F3 | Blue | 0 |
F4 | Blue | 0 |
F5 | Dark orange | 9 |
F6 | Yellow | 23 |
Blank | Blue | 0 |
The addition of a siderophore to the CAS solution causes a change in color from dark blue to orange-yellow. Activity against V. anguillarum is measured as the diameter of inhibition zones.