FIG 3.

Deletion of fleQ reveals the presence of a third Mn(II) oxidase, MopA. (A) Strains were streaked onto Lept medium and grown at RT for 7 days. Inset shows colonies after 10 μl leucoberbelin blue was dropped on top. (B) Quantification of Mn(III, IV) oxides in liquid cultures after shaking at RT for 3 days. Bars represent the average of three replicates; crosses indicate individual values. Strains shown: 1, wild type; 2, ΔmnxR; 3, ΔfleQ; 4, ΔmnxG ΔmcoA ΔfleQ; 5, ΔmnxG ΔmcoA ΔfleQ ΔmopA; 6, ΔmnxR ΔfleQ; 7, ΔmnxG ΔmcoA ΔfleQ ΔmopA + pUCP22; 8, ΔmnxG ΔmcoA ΔfleQ ΔmopA + pKG178.