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Native PAGE analysis results for protein stain (A); activity assay using gel incubated in 10 mM HEPES (pH 7.5) + 100 μM MnCl2 overnight at RT (B); activity assay using gel described for panel B after soaking in 0.0032% LBB (C). Lane 1, PAGERuler unstained protein ladder; lane 2, wild type; lane 3, MopA-OE; lane 4, MopA-KO + EV; lane 5, MopA-KO + pmopA.
