. 2016 Jun 13;82(13):3913–3927. doi: 10.1128/AEM.00271-16

TABLE 1.

PCR primers used in this study for stx and eae subtyping by sequencing

Gene	Primer designation	Primer sequence (5′–3′)	Fragment size (bp)	Reference or source
stx₂	F4	GGCACTGTCTGAAACTGCTCCTGT	627	7
stx₂	R1	ATTAAACTGCACTTCAGCAAATCC	627	7
	F4-f	CGCTGTCTGAGGCATCTCCGCT	625
	R1-e/f	TAAACTTCACCTGGGCAAAGCC	625
eae^a	EAE-F	ATTACTGAGATTAAGGCTGAT	682	51
eae^a	EAE-RB	ATTTATTTGCAGCCCCCCAT	682	51
eae-ε	EAE-F	ATTACTGAGATTAAGGCTGAT	722	51
eae-ε	LP5	AGCTCACTCGTAGATGACGGCAAGCG	722	51
eae-η	EAE-F	ATTACTGAGATTAAGGCTGAT	712	51
eae-η	LP8	TAGATGACGGTAAGCGAC	712	51
eae-ξ	EAE-F	ATTACTGAGATTAAGGCTGAT	468	51
eae-ξ	B49R	ACCACCTTTAGCAGTCAATTTG	468	51
eae-μ	FV373F	CAACGGTAAGTCTCAGACAC	443	51
eae-μ	FV373R	CATAATAAGCTTTTTGGCCTACC	443	51
eae-ν	IH1229aF	CACAGCTTACAATTGATAACA	311	51
eae-ν	IH1229aR	CTCACTATAAGTCATACGACT	311	51
eae^b	eae-F1	ACTCCGATTCCTCTGGTGAC	∼1,800–2,100, depending on the allele	75
eae^b	escD-R1	GTATCAACATCTCCCGCCCA	∼1,800–2,100, depending on the allele	75
eae^b	cesT-F3	CAGGAGCACAATCGCTGTTG	1,727	This study
eae^b	eae-R3	CAGACGATACGATCCAGACC	1,727	This study

Universal primers targeting the 3′ variable regions of the eae-α1, -α2, -β1, -β2, -γ2, -θ, -δ, -κ, -ζ, and -ι subtypes.

Primers targeting overlapping DNA fragments of the eae gene.