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. 2016 Jun 13;82(13):3913–3927. doi: 10.1128/AEM.00271-16

TABLE 2.

Distribution of STEC and EPEC strains by serotype and virulence gene profilesa,b

Strain no. Serotypea Sampleb
E. coli pathogroup Virulence gene subtype
Profile no. Total no. of genes detected No. of genetic markers in the following groupc:
Phylogroupa,d STa (no. of strains)
Type Site Date Origin stx1 stx2 eae Adhesion (n = 20) Type III secretion system (n = 30) Toxin (n = 12) Other function (n = 13) OI-71 (n = 5) OI-122 (n = 5) OI-57 (n = 6) OI-43/48 (n = 3) HPI (n = 2) EHEC markers (n = 4) nle genes (n = 13)
78 O26:H11 Shellfish 1 November 2013 M1.2 STEC 1a β1 1 47 8 25 3 9 5 4 6 3 2 4 12 B1 21
95 O91:H21 Freshwater 3 May 2013 W3.2 STEC 1a 2a, 2c, 2d 2 15 8 0 2 1 0 1 0 1 0 0 0 B1 442
59 O130:H11 Freshwater 1 December 2013 W1.1 STEC 1a 2a 3 12 7 1 2 0 0 0 0 1 0 0 0 B1 ND
14 ONT:H11 Freshwater 1 May 2013 W1.1 STEC 1a 2a 4 11 7 0 2 0 0 0 0 1 0 0 0 B1 295
163 O63:H6 Freshwater 1 November 2014 W1.1 STEC 2a 5 10 7 0 2 0 0 0 0 1 0 0 0 B1 2105
124 ONT:H10 Freshwater 2 April 2014 W2.1 STEC 1a 6 9 4 0 3 1 0 0 0 0 0 0 0 B1 1258
88 O185:H28 Freshwater 2 April 2013 W2.1 STEC 1a 2a 7 8 5 0 1 0 0 0 0 1 0 0 0 B2 658
72 O2:H32 Seawater 1 February 2014 W1.5 STEC 2e 8 6 2 0 1 2 0 0 1 1 0 0 0 A 10
126 O8:H19 Freshwater 3 July 2014 W3.1 STEC 2a, 2d 9 5 3 0 0 0 0 0 0 0 0 0 0 B1 162
16 O154:H31 Shellfish 1 May 2013 O1.1 STEC 1d 10 5 3 0 0 1 0 0 0 0 0 0 0 D 1892
19 O15:H16 Shellfish 1 June 2013 M1.1 STEC 2g 11 5 2 0 2 0 0 0 0 0 0 0 0 A 325
127 O76:H19 Freshwater 2 August 2014 W2.3 STEC 1d 12 5 2 0 2 0 0 0 0 0 0 0 0 B1 675
LR2 O100:H21 Shellfish 6 January 2003 M6.1 STEC 1d 13 4 3 0 0 0 0 0 0 0 0 0 0 ND ND
15 O154:H31 Freshwater 1 May 2013 W1.1 STEC 1d 14 4 3 0 0 0 0 0 0 0 0 0 0 D 1892
44 O154:HNM Shellfish 1 November 2013 M1.1 STEC 1d 14 4 3 0 0 0 0 0 0 0 0 0 0 D 1892
20 O100:HNM Shellfish 2 May 2013 M2.1 STEC + 15 3 1 0 0 1 0 0 0 1 0 0 0 A 993
24 O100:HNM Freshwater 3 May 2013 W3.1 STEC + 15 3 1 0 0 1 0 0 0 1 0 0 0 A 993
31 O100:HNM Shellfish 3 May 2013 O3.1 STEC + 15 3 1 0 0 1 0 0 0 1 0 0 0 A 993
32 O100:HNM Shellfish 3 May 2013 M3.1 STEC + 15 3 1 0 0 1 0 0 0 1 0 0 0 A 993
33 O100:HNM Shellfish 2 May 2013 C2.1 STEC + 15 3 1 0 0 1 0 0 0 1 0 0 0 A 993
35 O100:HNM Shellfish 3 June 2013 M3.1 STEC + 15 3 1 0 0 1 0 0 0 1 0 0 0 A 993
36 O100:HNM Sediment 3 June 2013 S3.1 STEC + 15 3 1 0 0 1 0 0 0 1 0 0 0 A 993
122 O100:HNM Shellfish 2 March 2014 M2.1 STEC + 15 3 1 0 0 1 0 0 0 1 0 0 0 A 993
125 O100:HNM Shellfish 3 June 2014 O3.1 STEC + 15 3 1 0 0 1 0 0 0 1 0 0 0 A 993
PB4 O38:H26 Shellfish 5 July 2003 M5.1 STEC 1c 16 3 1 0 1 0 0 0 0 0 0 0 0 ND ND
1 O149:H1 Freshwater 1 April 2013 W1.1 STEC 1d 17 2 1 0 0 0 0 0 0 0 0 0 0 D 132
3 O149:HNM Freshwater 1 April 2013 W1.1 STEC 1d 17 2 1 0 0 0 0 0 0 0 0 0 0 D 132
73 O28:H1 Shellfish 1 March 2014 C1.1 STEC 1d 17 2 1 0 0 0 0 0 0 0 0 0 0 D 132
SP5 O157:H7 Shellfish 4 September 2003 O4.1 EPEC γ1 1 50 8 30 3 8 5 5 5 3 0 3 13 ND ND
77 O145:H28 Shellfish 2 June 2013 C2.1 EPEC γ1 2 39 4 24 3 7 3 5 2 3 0 3 7 D ND
79 O145:H28 Shellfish 2 June 2013 M2.1 EPEC γ1 2 39 4 24 3 7 3 5 2 3 0 3 7 D ND
121 O103:H25 Freshwater 3 March 2014 W3.1 EPEC θ 2 35 5 19 3 7 3 5 3 2 0 4 7 B1 343
96 O103:H2 Freshwater 2 June 2013 W2.3 EPEC θ 3 35 5 19 3 7 3 5 3 2 0 4 7 B1 343
120 O153:H2 Freshwater 2 March 2014 W2.3 EPEC ε 4 34 5 19 2 7 1 5 2 2 2 2 8 B1 27
123 O153:H2 Shellfish 3 March 2014 M3.1 EPEC ε 4 34 5 19 2 7 1 5 2 2 2 2 8 B1 27
81 O26:H11 Shellfish 1 November 2013 M1.2 EPEC β1 5 34 5 20 3 5 5 4 3 1 2 0 10 B1 29
84 O26:H11 Freshwater 1 November 2013 W1.3 EPEC β1 5 34 5 20 3 5 5 4 3 1 2 0 10 B1 29
85 O26:H11 Shellfish 1 November 2013 C1.1 EPEC β1 5 34 5 20 3 5 5 4 3 1 2 0 10 B1 29
102 O23:H8 Freshwater 2 August 2013 W2.1 EPEC θ 6 33 5 22 2 3 5 5 5 0 0 0 11 B1 327
129 O23:H8 Shellfish 2 August 2014 C2.1 EPEC θ 7 32 5 22 2 2 5 4 5 0 0 0 11 B1 327
80 O26:H11 Freshwater 2 August 2013 W2.1 EPEC β1 8 27 4 17 2 3 2 4 2 1 2 0 8 B1 29
64 O39:HNM Freshwater 1 January 2014 W1.3 EPEC ι 9 25 3 19 1 1 3 0 5 0 0 0 10 B1 39
111 ONT:H31 Freshwater 1 October 2014 W1.3 EPEC θ 10 24 3 16 3 1 1 3 2 0 0 0 6 B1 40
131 O15:H2 Freshwater 3 August 2014 W3.2 EPEC β1 11 23 3 17 1 1 2 5 2 0 0 0 8 B1 20
153 O28:HNM Freshwater 1 June 2014 W1.1 EPEC γ1 12 23 0 15 2 5 1 0 2 2 0 3 2 D 137
61 O153:H21 Freshwater 1 January 2014 W1.1 EPEC θ 13 22 3 17 1 0 2 3 1 0 0 0 7 B1 40
115 O108:H21 Freshwater 3 November 2014 W3.1 EPEC θ 13 22 3 17 1 0 2 3 1 0 0 0 7 B1 337
117 O108:H21 Shellfish 3 November 2014 W3.2 EPEC θ 13 22 3 17 1 0 2 3 1 0 0 0 7 B1 337
118 O108:H21 Shellfish 2 January 2014 C2.1 EPEC θ 13 22 3 17 1 0 2 3 1 0 0 0 7 B1 337
134 O146:H21 Shellfish 3 August 2014 M3.1 EPEC θ 14 22 3 17 1 0 2 3 1 0 0 0 7 B1 442
65 O40:HNM Freshwater 1 February 2014 W1.1 EPEC β1 15 22 0 16 1 4 3 0 1 1 2 2 6 A 10
162 ONT:H8 Freshwater 1 August 2014 W1.2 EPEC θ 16 21 4 15 1 0 1 3 1 0 0 0 5 B1 327
49 O98:H8 Shellfish 1 November 2014 M1.1 EPEC ι 17 21 5 15 0 0 1 1 0 0 0 1 5 B1 590
159 O91:H10 Shellfish 1 July 2014 C1.1 EPEC β2 18 21 3 14 1 2 1 0 1 0 2 0 4 B2 641
139 O88:H25 Shellfish 3 September 2014 M3.1 EPEC β1 19 21 4 15 1 0 1 4 1 0 0 0 6 B1 328
154 ONT:H8 Freshwater 1 April 2014 W1.2 EPEC θ 20 20 3 15 1 0 1 3 1 0 0 0 5 B1 327
105 O71:HNM Freshwater 1 August 2014 W1.2 EPEC η 21 20 5 14 0 0 2 0 0 0 0 1 5 A 517
133 O88:H8 Shellfish 3 August 2014 O3.1 EPEC ι 22 20 2 17 0 0 3 0 1 0 0 1 7 B1 590
136 O146:H6 Shellfish 2 September 2014 C2.1 EPEC θ 23 20 3 15 1 0 1 3 1 0 0 0 5 B1 442
37 O167:H3 Freshwater 1 August 2013 W1.1 EPEC β1 24 20 0 17 0 2 5 0 2 0 0 0 7 D 2558
113 O128:H2 Freshwater 2 November 2014 W2.3 EPEC β1 25 19 3 13 0 2 1 0 0 0 2 1 3 B1 20
132 O113:H6 Shellfish 3 August 2014 O3.1 EPEC β2 26 19 0 14 2 2 1 0 1 0 2 0 4 B2 121
160 ONT:H6 Freshwater 1 August 2014 W1.1 EPEC β2 27 19 1 15 0 2 2 0 1 0 2 0 5 B2 28
86 O5:H40 Shellfish 2 February 2014 M2.1 EPEC θ 28 19 1 17 0 0 3 2 0 0 0 0 7 A 10
128 O86:H31 Shellfish 2 August 2014 C2.1 EPEC θ 29 19 3 14 1 0 2 3 0 0 0 1 4 D 2569
135 O125:H6 Freshwater 2 September 2014 W2.3 EPEC α2 30 19 1 15 2 0 2 0 0 0 0 1 4 B2 583
152 ONT:H6 Seawater 1 December 2014 W1.5 EPEC β2 31 18 1 13 0 3 1 0 1 1 2 0 4 B2 28
54 O125:H6 Freshwater 1 November 2014 W1.3 EPEC α2 32 18 1 15 1 0 2 0 0 0 0 0 4 B2 583
116 O113:H6 Freshwater 3 November 2014 W3.2 EPEC β2 33 18 0 14 1 2 1 0 1 0 2 0 4 B2 121
138 ONT:H6 Freshwater 3 September 2014 W3.2 EPEC β2 34 18 1 14 0 2 2 0 1 0 2 0 5 B2 28
149 O159:H7 Freshwater 1 November 2014 W1.3 EPEC ε 35 18 3 14 0 0 2 0 0 0 0 1 4 B1 ND
104 O157:H16 Freshwater 1 July 2014 W1.4 EPEC ε 36 18 0 17 0 0 3 0 4 0 0 0 10 A 10
58 O63:H6 Freshwater 2 November 2014 W2.1 EPEC α2 37 17 1 14 0 1 1 0 1 1 0 0 3 B2 122
62 O63:H6 Freshwater 1 January 2014 W1.2 EPEC α2 37 17 1 14 0 1 1 0 1 1 0 0 3 B2 122
142 O63:H6 Freshwater 3 October 2014 W3.2 EPEC α2 37 17 1 14 0 1 1 0 1 1 0 0 3 B2 122
144 O63:HNM Freshwater 3 October 2014 W3.2 EPEC α2 37 17 1 14 0 1 1 0 1 1 0 0 3 B2 122
141 ONT:H6 Freshwater 3 October 2014 W3.2 EPEC α2 37 17 1 14 0 1 1 0 1 1 0 0 3 B2 122
43 O51:HNM Freshwater 2 October 2013 W2.2 EPEC α1 38 17 1 14 0 1 1 0 1 1 0 0 4 B2 589
51 O113:H6 Shellfish 1 November 2013 M1.2 EPEC β2 39 17 0 14 0 2 1 0 1 0 2 0 4 B2 121
60 O2:H45 Freshwater 2 December 2013 W2.1 EPEC κ/δ 40 17 2 13 1 0 2 0 0 0 0 0 3 B2 1907
63 O179:H31 Freshwater 1 January 2014 W1.2 EPEC ζ 41 17 1 13 0 2 1 0 0 0 2 0 4 B2 1092
107 O25:H2 Freshwater 1 September 2014 W1.1 EPEC β1 42 17 2 14 0 0 1 0 2 0 0 0 5 B1 20
38 ONT:H2 Freshwater 1 August 2013 W1.2 EPEC β1 42 17 2 14 0 0 1 0 2 0 0 0 5 B1 20
47 O63:H6 Freshwater 1 November 2013 W1.4 EPEC α2 43 17 1 13 2 0 1 0 0 0 0 0 3 B2 583
42 O29:H19 Freshwater 2 October 2013 W2.1 EPEC ε 44 16 3 12 0 0 1 0 0 0 0 1 2 B1 517
46 O116:H20 Freshwater 1 November 2013 W1.3 EPEC β1 45 16 1 14 0 0 2 0 0 0 0 0 5 A 10
50 O28:H16 Shellfish 1 November 2013 M1.1 EPEC β1 45 16 1 14 0 0 2 0 0 0 0 0 5 A 10
52 O113:H6 Shellfish 1 November 2013 C1.1 EPEC β2 46 16 0 13 0 2 1 0 1 0 2 0 3 B2 1583
161 O137:H6 Freshwater 1 August 2014 W1.1 EPEC β2 47 16 1 12 0 2 1 0 0 0 2 0 4 B2 2678
39 ONT:H6 Freshwater 1 September 2013 W1.3 EPEC β2 48 16 1 12 0 2 1 0 1 0 2 0 4 B2 28
67 O33:H6 Freshwater 1 February 2014 W1.3 EPEC β2 49 15 1 10 0 3 0 0 0 1 2 0 0 B2 28
112 O85:H31 Freshwater 1 October 2014 W1.3 EPEC ζ 50 13 0 10 0 2 0 0 0 0 2 0 1 B2 803
71 O33:H6 Freshwater 1 February 2014 W1.3 EPEC β2 51 13 0 10 0 2 0 0 0 0 2 0 0 B2 28
158 O20:HNT Seawater 1 April 2014 W1.5 EPEC θ 52 12 0 10 1 0 0 0 0 0 0 0 0 A 3747
164 O98:H56 Freshwater 1 December 2014 W1.4 EPEC θ 52 12 0 10 1 0 0 0 0 0 0 0 0 A ND
70 O98:HNM Seawater 1 February 2014 W1.5 EPEC θ 52 12 0 10 1 0 0 0 0 0 0 0 0 A 3747
150 O98:HNT Freshwater 1 December 2014 W1.4 EPEC θ 52 12 0 10 1 0 0 0 0 0 0 0 0 A 3747
40 O71:H49 Shellfish 1 October 2013 C1.1 EPEC κ/δ 53 12 0 11 0 0 0 0 0 0 0 0 2 B2 2813
41 O71:H49 Shellfish 1 October 2013 M1.2 EPEC κ/δ 53 12 0 11 0 0 0 0 0 0 0 0 2 B2 2813
48 O8:H14 Freshwater 1 November 2013 W1.4 EPEC κ/δ 53 12 0 11 0 0 0 0 0 0 0 0 2 B2 4178
106 O85:HNM Freshwater 1 August 2014 W1.4 EPEC ζ 54 12 0 9 0 2 0 0 0 0 2 0 1 B2 803
74 O98:HNM Freshwater 1 March 2014 W1.4 EPEC θ 55 11 0 9 1 0 0 0 0 0 0 0 0 A 3747
103 O145:H34 Freshwater 1 July 2014 W1.3 EPEC ι 56 11 0 10 0 0 0 0 0 0 0 0 2 B2 526
a

The serotype, phylogroup, and ST (MLST sequence type) were determined previously by Balière et al. (47) and Gourmelon et al. (48).

b

Sampling sites are coded as follows: 1, La Fresnaye, Brittany; 2, Regnéville sur mer, Normandy; 3, La Vanlée, Normandy; 4, Saint-Pol-de-Léon, Brittany; 5, Pirou, Normandy; 6, Sèvre, Poitou-Charente region. Freshwater samples are coded as follows: W1.1, Frémur (lat 48.3711280, long −2.208756); W1.2, Le Rat (lat 48.650571, long −2.1912390); W1.3, Le Clos (lat 48.3728867, long −2.1736788); W1.4, Kermiton (lat 48.3738516, long −2.1716267); W2.1, La Sienne (lat 49.0195333, long −1.5055472); W2.2, La Soulles (lat 49.0234806, long −1.4890194); W2.3, Regnéville sur mer (lat 48.9989694, long −1.5585417); W3.1, La Vanlée (lat 48.9147833, long −1.5498417); W3.2, Les Hardes (lat 48.9309028, long −1.5350306). Seawater samples are coded as W1.5 (Kermiton [lat 48.627192, long −2.291158]). Samples from cockles are coded as C1.1 (Cockles La Fresnaye [lat 48.3750614, long −2.1716026]) or C2.1 (Cockles Regnéville sur mer [lat 48.9716028, long −1.5648194]). Samples from mussels are coded as follows: M1.1, Mussels1 La Fresnaye (lat 48.3825912, long −2.1731444); M1.2, Mussels2 La Fresnaye (lat 48.3812179, long −2.175184); M2.1, Mussels Regnéville sur mer (lat 48.9716444, long −1.5748083); M3.1, Mussels La Vanlée (lat 48.9303583, long −1.5939472); M5.1, Mussels Pirou (lat 49.182884, long −1.6146958); M6.1, Mussels Sèvre (lat 46.29665, long −1.1457804). Samples from oysters are coded as follows: O1.1, Oysters 1 La Fresnaye (lat 48.3820423, long −2.1738512); O1.2, Oysters 2 La Fresnaye (lat 48.388116, long −2.1713438); O3.1, Oysters La Vanlée (lat 48.9303583, long −1.5939472); O4.1, Oysters Saint-Pol-de-Léon (lat 48.674128, long −3.970621). Sediment samples are coded as S3.1 (Sediment La Vanlée [lat 48.9147833, long −1.5498417]).

c

Genetic marker groups are as follows. The adhesion group includes eibG, iha, saa, toxB, orfA, orfB, paa, stcE, sab, efa1 or lifA, bfpA, espP, the F6/F987P gene (fasA), the F18/F107 gene (fedA), the F41 gene (fimF41a), lpfAO157, lpfAO26, lpfAO113, ehaA, and epeA. The type III secretion system group includes espB, espD, espA, tir, espZ, escC, espG, escD, escN, escV, escJ, nleB, nleE, nleF, nleG, nleH1-2, nleA, espM1, nleC, nleD, nleH1-1, nleG2, nleG5-1, nleG5-2, nleG6-2, espV, espK, espN, espJ, and espM2. The toxin group includes subA, ent, ehxA, cdt-I, cdt-III, cdt-V, sta (estla), lt (elt), hlyA, cnf1, cnf2, and astA. The “other function” group includes katP, ecf1, pagC, terE, ureD, aggR, pic, irp2, fyuA, Z2098, ECs1763, ECs1822, and etpD. The OI-71 pathogenicity island includes nleF, nleG, nleH1-2, nleA, and espM1; OI-122 includes efa1 or lifA, nleB, nleE, ent, and pagC; OI-57 includes nleG2, nleG5-1, nleG5-2, nleG6-2, Z2098, and ECs1763; OI-43/48 includes iha, terE, and ureD; the HPI includes irp2 and fyuA. EHEC markers include espV, espK, ureD, and Z2098. The nle gene group includes nleB, nleE, nleF, nleG, nleH1-2, nleA, nleC, nleD, nleH1-1, nleG2, nleG5-1, nleG5-2, and nleG6-2.

d

ND, not done.