TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Characteristics or purpose | Reference or source |
|---|---|---|
| Xanthomonas oryzae pv. oryzae strains | ||
| MAFF311018 | Wild type, Japanese strain | |
| 74Hpa1::Lux | Bioluminescent strain with a lux operon controlled by the hpa1 promoter | 26 |
| Clone no. 34 | 74Hpa1::Lux-derived mutant with transposon EZ::TN <KAN2> inserted at position +38 of XOO_2767 (gamR) | This study |
| ΔGamR strain | gamR deletion mutant, lacking an internal 834-bp region (+25 to +858) in the 972-bp coding region | This study |
| Escherichia coli strains | ||
| DH5α MCR | Strain used for cloning | Stratagene |
| BL21(DE3)(pLysS) | Strain used for protein expression and purification | Merck |
| Plasmids | ||
| pBluescript II SK+ | Plasmid vector, Ampr | Stratagene |
| pBSΔGamR::Kmr/sac | Plasmid used for the gamR deletion mutant, containing the upstream and downstream regions of gamR with the kanamycin resistance gene and levansucrase gene | This study |
| pET28b | Plasmid vector used in E. coli protein expression system | Merck |
| pETHis-GamR | Coding region of gamR cloned in pET28b | This study |
| pHM1 | Broad-host-range vector, pSa ori, Spr | 34 |
| pHMGamR | Complementation plasmid for ΔGamR strain | This study |
| pHMHrpG::GUS-W | Promoterless gus gene preceded by −803 to +130 of hrpG cloned in pHM1 | This study |
| pHMHrpG::GUS-M4 | Plasmid similar to pHMHrpG::GUS-W but with base substitutions at the GamR-binding sequence | This study |
| pHMHrpX::GUS-W | Promoterless gus gene preceded by −891 to +42 of hrpX cloned in pHM1 | This study |
| pHMHrpX::GUS-M4 | Plasmid similar to pHMHrpX::GUS-W but with base substitutions at the GamR-binding sequence | This study |
| pHMXOO2768::GUS-W | Promoterless gus gene preceded by −439 to +92 of hrpG cloned in pHM1 | This study |
| pHMXOO2768::GUS-M1 | Plasmid similar to pHMXOO2768::GUS-W but with base substitutions at the GamR-binding sequence | This study |
| pHMGamR::GUS | Promoterless gus gene preceded by −504 to +22 of gamR cloned in pHM1 | This study |