FIG 2.

Phylogenetic analysis of the HAV strains isolated in raw and treated wastewater samples from five STPs in Tunisia during 13 months of study. Wastewater sample codes: KR, Kairouan; SS, Sousse-South; D, Dekhila; SH, Sahline; J, Jammel. The phylogenetic tree was constructed using 223-bp sequences of the VP3-VP1 region and the NJ algorithm with distance calculation by Kimua-2-parameter correction (MEGA6). Only bootstrap values of >50% are shown on the phylogenetic tree. Bar, 0.02 substitutions per nucleotide position. Sequences obtained in the present study were deposited in the GenBank database.