TABLE 1.
Specific and relative activities for a range of amine donors and acceptors
| Substrate category and namea | Sp act (nmol/min/mg) | Relative activityb (%) |
|---|---|---|
| Amine donors | ||
| ω-Aminoalkanoates | ||
| Glycine | 9.9 ± 2.4 | 1.4 |
| β-Alanine | 378.3 ± 7.4 | 54.1 |
| 4-Aminobutyric acid | 635.4 ± 9.9 | 90.8 |
| 5-Aminopentanoic acid | 368.4 ± 1.9 | 52.7 |
| 6-Aminohexanoic acid | 437.6 ± 1.3 | 62.5 |
| 7-Aminoheptanoic acid | 482.1 ± 2.5 | 68.9 |
| 8-Aminooctanoic acid | 699.7 ± 9.9 | 100.0 |
| 12-Aminododecanoic acid | 126.1 ± 2.5 | 18.0 |
| Diamines | ||
| 1,3-Diaminopropane | 24.7 ± 0.7 | 3.5 |
| Putrescine | 267.0 ± 1.7 | 38.2 |
| Cadaverine | 603.0 ± 0.8 | 86.2 |
| 1,6-Hexamethylenediamine | 388.2 ± 1.7 | 55.5 |
| 1,7-Heptanediamine | 346.2 ± 2.5 | 49.5 |
| 1,8-Octaneamine | 279.4 ± 2.5 | 39.9 |
| 1,9-Nonanediamine | 212.6 ± 2.5 | 30.4 |
| 1,10-Decanediamine | 679.9 ± 7.4 | 97.2 |
| Amino acids | ||
| l-Ornithine | 4.9 ± 0.3 | 0.7 |
| d-Ornithine | 27.2 ± 0.3 | 3.9 |
| l-Lysine | 2.5 ± 0.2 | 0.4 |
| d-Lysine | 61.8 ± 0.4 | 8.8 |
| l-Arginine | 4.9 ± 0.4 | 0.7 |
| d-Arginine | NDc | ND |
| l-Glutamic acid | ND | ND |
| Citrulline | ND | ND |
| Other amine donors | ||
| N-Acetyl-l-Ornithine | ND | ND |
| 4-Amino-2-(S)-hydroxybutyric acid | 336.3 ± 2.5 | 48.1 |
| 5-Aminolevulinic acid | ND | ND |
| Serinol | 9.9 ± 0.2 | 1.4 |
| d/l-Aminoisobutyric acid | 435.2 ± 2.5 | 62.2 |
| Taurine | 7.4 ± 0.5 | 1.1 |
| Creatine | ND | ND |
| 3-Aminocyclohexanoic acid | 12.4 ± 0.7 | 1.8 |
| 3-Aminoheptanoic acid | 2.5 ± 0.5 | 0.4 |
| (S)-2,4-Diaminobutyric acid | ND | ND |
| β-Homoleucine | ND | ND |
| 2,6-Diaminopimelate | ND | ND |
| Aminomethylphosphonic acid | ND | ND |
| 2-Aminoindane | 24.7 ± 0.6 | 3.5 |
| 2-Methylbenzylamine | 54.4 ± 4.9 | 7.8 |
| Isopropylamine | 232.4 ± 1.3 | 33.2 |
| Cyclohexylamine | 42.0 ± 0.4 | 6.0 |
| 6-Aminohexanol | 489.6 ± 2.4 | 70.0 |
| Amine acceptors | ||
| Propionaldehyde | 202.0 ± 7.2 | 68.3 |
| Butyraldehyde | 295.8 ± 7.2 | 100.0 |
| Heptanal | 21.6 ± 1.8 | 7.3 |
| Octanal | 24.1 ± 2.1 | 8.1 |
| Dodecanal | 40.9 ± 19.2 | 13.8 |
| Glyceraldehyde | 101.0 ± 2.4 | 34.1 |
| Cyclohexanone | ND | ND |
| HMF | 40.9 ± 1.1 | 13.8 |
a
For each amine donor substrate, the acceptor used was pyruvate (assay conditions included potassium phosphate buffer at 100 mM, pH 10). For each amine acceptor substrate, the donor used was L-alanine (assay conditions included potassium phosphate buffer at 100 mM, pH 8).
b
Relative activities are in comparison with 8-aminooctanoic acid for the various amine donors and with butyraldehyde for the amine acceptors.
c
ND, activity not determined.