TABLE 2.
Substrate | R414K sp act (nmol/min/mg) | Relative activity (% of WT) |
---|---|---|
β-Alanine | 75.1 ± 1.0 | 19.9 |
12-Aminododecanoic acid | ND | ND |
Putrescine | 38.6 ± 2.0 | 14.4 |
Cadaverine | 57.9 ± 1.0 | 9.6 |
1,3-Diaminopropane | 5.1 ± 1.0 | 20.5 |
Note that for each substrate, the acceptor used was pyruvate. Assay conditions are described in Materials and Methods (and included potassium phosphate buffer at 100 mM, pH 10). ND, activity not determined.