TABLE 1.
Analysis of L. rhamnosus colonies for the presence of the spa CBA-srtC1 pilus gene cluster
| Estimated no. of generations and growth condition | srtC1 PCR screening (no. of positive isolates/total no. of isolates)a |
|---|---|
| 100 generations | |
| Control (MRS only) | 10/10 |
| 3% (wt/vol) NaCl | 10/10 |
| 30-s vortex treatment | 10/10 |
| 0.25% (wt/vol) bile salts | 10/10 |
| 1,000 generations | |
| Control (MRS only) | 19/20 |
| 3% (wt/vol) NaCl | 20/20 |
| 30-s vortex treatment | 13/20 |
| 0.25% (wt/vol) bile salts | 0/20 |
a
Isolates were screened after approximately 100 and 1,000 generations by conventional endpoint PCR using srtC1-specific primers (see Materials and Methods).