FIG 1.

Growth inhibition of EGD-e by blue light. EGD-e was illuminated with blue light (460 to 470 nm, 1.5 to 2.0 mW cm⁻²) either on BHI agar (A) or in a BHI liquid culture (B). (B) White bars represent growth following continuous illumination, and the black bars represent a dark control. The top graph shows the final OD₆₀₀ after 24 h, and the bottom graph shows the difference in lag times between the two conditions. Overnight cultures were standardized to an OD₆₀₀ of 1.0 and diluted to 10⁻⁵ (approximately 10⁴ cells ml⁻¹). Cells were incubated at 30°C for 24 h. The values represent the means of the results from three independent replicates. The error bars represent the standard deviations between replicates. Student's t test was carried out to determine the statistical difference (P ≤ 0.05, indicated with an asterisk) between cultures grown in light and dark.