FIG 3.

Lack of Reg2 does not lead to constitutive accumulation of phospho-Thr210-Snf1. (A) Cells of the indicated genotypes were grown to mid-log phase in YEP containing 2% glucose (H, high glucose) followed by a shift for 1 h to YEP containing 0.05% glucose (L, low glucose). (B and C) Cells were grown as for panel A, and the glucose-limited cells were then replenished with abundant (2%) glucose for 1 min (+1′). The levels of phospho-Thr210-Snf1 (P-T210) and total Snf1 protein (Total) were analyzed by immunoblotting. The strains were MMY128 (WT) and MMY18 (reg2Δ).