FIG 5.

Overexpression of Reg2 has no significant effect on the Thr210 phosphorylation status of Snf1 in low glucose. (A) Wild-type cells (MKY343) transformed with pLexA-Reg2 (LexA-Reg2, +) or with the corresponding vector pEG202 (LexA-Reg2, −) were grown to mid-log phase in SC medium lacking histidine and containing 2% glucose (H, high glucose) and then shifted to an otherwise identical medium containing 0.05% glucose (L, low glucose). The levels of LexA-Reg2, phospho-Thr210-Snf1 (P-T210), and total Snf1 protein (Total) were analyzed by immunoblotting. (B) Expression of LexA-Reg2 in 2% glucose (H, high glucose) and in 0.05% glucose (L, low glucose). Cells of MKY343 (WT) transformed with pLexA-Reg2 were grown as for panel A.