FIG 2.

Antibody binding to Env on the surfaces of virus-infected cells. CEM.NKR-_CCR5-sLTR-Luc cells infected with HIV-1_NL4-3, HIV-1_JR-FL, or SHIV_AD8-EO were stained with HIV-1 Env-specific antibodies followed by a PE-conjugated anti-human IgG F(ab′)₂. The cells were also stained for surface expression of CD45 and CD4, intracellular expression of the viral Gag protein, and with a viability dye. The histograms show Env staining on virus-infected (Gag⁺ CD4^low) cells of the viable CD45⁺ population. The shaded area indicates nonspecific staining with the DEN3 control antibody. Max, maximum.