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Comparison of ADCC activity and Env binding by HIV-1 Env-specific antibodies. Partial area under the curve values (pAUC) for ADCC activity were calculated from percent RLU measurements at the four highest antibody concentrations tested, as previously described (25, 41). pAUC values for HIV-1NL4-3, HIV-1JR-FL, and SHIVAD8-EO were compared to the geometric mean fluorescence intensities (gMFIs) of Env staining on the surfaces of virus-infected cells by Spearman correlation.
