FIG 4.

Neutralization of HIV-1_NL4-3, HIV-1_JR-FL, and SHIV_AD8-EO by Env-specific antibodies. HIV-1 Env-specific monoclonal antibodies were tested for the ability to block viral infectivity. HIV-1_NL4-3, HIV-1_JR-FL, and SHIV_AD8-EO were incubated with serial dilutions of each antibody for 1 h before addition to TZM-bl reporter cells. Three days postinfection, neutralization was calculated from the luciferase activity (RLU) in TZM-bl cell lysates for cells inoculated with virus plus antibody relative to cells inoculated with virus in the absence of antibody. The error bars indicate standard deviations of the means for triplicate wells, and the dotted line indicates half-maximal infection or 50% neutralization.