TABLE 3.
Env staining on the surface of virus-infected cellsa
| Antibody | gMFI of Env staining |
||
|---|---|---|---|
| HIV-1NL4-3 | HIV-1JR-FL | SHIVAD8-EO | |
| DEN3 | 391 | 361 | 311 |
| b12 | 5,370 | 1,179 | 355 |
| b6 | 4,391 | 719 | 396 |
| VRC01 | 2,289 | 901 | 351 |
| PGV04 | 2,744 | 851 | 389 |
| 3BNC117 | 3,041 | 1,358 | 437 |
| PG9 | 872 | 466 | 313 |
| PG16 | 1,109 | 672 | 335 |
| PGT126 | 1,882 | 1,930 | 620 |
| PGT121 | 483 | 1,193 | 599 |
| 10-1074 | 1,951 | 1,191 | 540 |
| 2G12 | 6,167 | 1,148 | 411 |
| 2F5 | 800 | 482 | 333 |
| 4E10 | 1,768 | 756 | 341 |
| 10E8 | 1,836 | 619 | 349 |
| 17b | 4,451 | 473 | 382 |
| X5 | 1,743 | 412 | 322 |
| A32 | 3,073 | 642 | 446 |
| C11 | 2,108 | 504 | 396 |
| 240D | 5,205 | 533 | 373 |
| F240 | 4,996 | 560 | 402 |
| 98-6 | 1,438 | 473 | 327 |
| 126-7 | 1,740 | 471 | 331 |
a
CEM.NKR-CCR5-sLTR-Luc cells infected with HIV-1NL4-3, HIV-1JR-FL, or SHIVAD8-EO were stained with HIV-1 Env-specific antibodies followed by a PE-conjugated anti-human IgG F(ab′)2. The cells were also stained for surface expression of CD45 and CD4, intracellular expression of the viral Gag protein, and with a viability dye. Values indicate the geometric mean fluorescence intensity (gMFI) of Env staining on the surfaces of virus-infected (Gag+ CD4low) cells of the viable CD45+ population. DEN3 is a dengue virus-specific monoclonal antibody included as a control for nonspecific staining.