TABLE 5.

Possible biological relevance of some mutations detected in the Biysk isolates

Genome region	Nucleotide (amino acid) mutation	Known or assumed biological relevance (figure or reference)
5′ UTR	U156→C	Stabilization of domain II structure (Fig. 1A)
	U398→C	Stabilization of domain IV structure (Fig. 1B)
	A481→G	Deattenuation (41)
VP2	A1429→G (S159→G)	Region of antigenic site 2 (32)
	G1462→A (A170→T)	Antigenic site 2 (45)
	A1468→G (N172→D)	Antigenic site 2 (45)
VP3	A1997→G (H77→R)	Antigenic site 3b (45)
	C1998→A (H77→Q)	Antigenic site 3b (45)
	A2005→G (T80→A)	Antigenic site 3b (45)
VP1	U2909→C (I143→T)	Deattenuation (41)
	G3343→A (D288→N)	Antigenic site 3 (45)
3C	G5949→A	Stabilization of ciRNA structure (Fig. 1D)
3′ UTR	G7405→A	Stabilization of oriR structure (Fig. 1C)