FIG 1.

Genes differentially expressed in HPV-16 E7-expressing cells. (A) Numbers of genes differentially expressed between NIKS cells expressing E7 and a vector control, as determined by RNA-seq, are presented as columns. Blue, total number of protein-coding mRNAs detected; red, upregulated genes in E7-expressing cells; green, downregulated genes in E7-expressing cells. (B) Real-time PCR assay for selected genes in keratinocytes. Upper panel, relative expression levels of mRNAs in E7-expressing NIKS cells or PHKs compared with vector, measured by real-time PCR and RNA-seq. Lower panels, images of DNA gel electrophoresis. MWM, molecular weight marker.