Figure 3.

Effect of the Rho-associated kinase inhibitor Y27632 and the matrix metalloproteinase inhibitor Ro 28–2653 on the invasion capacity of phase III tumorigenic cells, mock-transfected cells (C1 and C2 clones) and Snail-expressing clones. Invasion was measured using Boyden chambers. Grey columns, Ro 28–2653 (0.1 mM); black columns, Y27632 (10 mM). Invasion of cells exposed to inhibitors is shown as percentage of invasion in untreated cells (white columns). Data are presented as the mean ± standard deviation of 2–4 independent experiments. In cen3tel, C1 and C2 cells, the P-value refers to Y27632-treated cells vs. control cells, while in Snail clones, the P-value refers to Ro 28–2653-treated cells vs. control cells (*P<0.05; **P<0.005; ***P<0.0005). WT, wild-type.