Figure 4.

In vivo tumorigenic and metastatic capacity of Snail-expressing clones. (A) Growth curves of tumors obtained in SCID mice (n=3) after subcutaneous inoculation of mock-transfected phase III tumorigenic cen3tel cells (C1) or Snail-expressing clones (Snail-wt 6 and 9). Vertical bars indicate the standard error. (B) Number of lung metastases obtained upon intravenous injection of mock-transfected phase III tumorigenic cen3tel cells (C1) or Snail-expressing clones (Snail-wt 6 and 9) into SCID mice. In two mice inoculated with Snail-wt 9 cells, the precise number of metastases could not be determined due to their large abundance, and was arbitrarily assigned a value of 200. Horizontal bars represent the median number of metastases. WT, wild-type; SCID, severe combined immunodeficiency.