Figure 4.

Fibulin-5 activates ERK in Ishikawa cells. (A) Ishikawa cells were treated with phosphate-buffered saline vehicle control (Con), or with 0.1 or 0.5 µg/ml recombinant human fibulin-5 (FBN5 0.1 and FNB5 0.5, respectively) and cell lysates collected after 10 min. Phosphorylated (p)ERK was detected by western blotting and total ERK was used as a loading control. (B) Densitometry was performed on n=2 blots at 10 min and pERK was normalised to ERK. Data are presented as the mean ± standard error of the mean. *P<0.05. ERK, extracellular signal-regulated kinase.