Figure 3.

PNPLA7 expression was restored after 5-Aza-docy treatment. (A) PNPLA7 mRNA expression in HepG2 cells with and without demethylation treatment. Data represent mean±sd from at least 3 independent experiments, **P<0.01. (B) Methylation status of the fragment was assessed using BSP in HepG2 cells with and without demethylation treatment. (C) Densitometric analysis of methylation rate of the tested fragment in HepG2 cells with and without demethylation treatment based on BSP results, **P<0.01. PNPL7, patatin-like phospholipase domain-containing protein 7; HCC, hepatocellular carcinoma; BSP, bisulfite sequencing PCR.