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. Author manuscript; available in PMC: 2016 Sep 28.
Published in final edited form as: Nat Biotechnol. 2016 Mar 28;34(5):547–555. doi: 10.1038/nbt.3520

Figure 1. Framework for noninvasive profiling of ctDNA.

Figure 1

(a) Theoretical ctDNA detection limits are shown for single-mutation non-invasive genotyping and multi-mutation monitoring based on a typical cfDNA yield from 10 mL blood (assuming ~50% molecule recovery8; top) (Methods). Background noise of CAPP-Seq is shown to increase as a function of lower ctDNA allele fractions, and was determined using pooled cfDNA sequencing data from 30 healthy adult controls (Methods). (b) Schematic illustrating the potential application of CAPP-Seq to noninvasive (biopsy-free) genotyping and monitoring of ctDNA.