Figure 2.

CCR2 and CXCR2 ligands are upregulated in inflamed, Brucella melitensis–infected joints. Joint homogenates (5 mice/group) from wild-type (WT) and interferon γ (IFN-γ)^−/− mice infected intraperitoneally with approximately 1 × 10⁵ colony-forming units of B. melitensis 16 M were assayed for T-cell–associated cytokines (A) inflammatory cytokines (B), and chemokines (C) by the Luminex assay 23 and 30 days after infection and normalized using a bicinchoninic acid protein assay. *P < .05 as compared to WT mice. Error bars depicted standard errors of the mean. Abbreviations: IL-1β, interleukin 1β; IL-2, interleukin 2; IL-4, interleukin 4; IL-6, interleukin 6; IL-10, interleukin 10; IL-13, interleukin 13; IL-17, interleukin 17; TNF-α, tumor necrosis factor α.