Fig 5. NK cells and memory T cells produce IFNγ in the acute response L. pneumophila.

IFNγ-YFP reporter mice (GREAT mice, A-F), IFNγ-YFP.IL12p35^-/- (A,D,E) or C57BL/6 mice (G) were infected with L. pneumophila and analysed on day 2 (A, D-F) or as indicated (B, C, G). A. YFP fluorescence in the cell types indicated. NK cells were defined as NK1.1⁺NKp46⁺CD3^-, T cells as CD3⁺TCRβ⁺ or CD3⁺TCRγδ⁺ and NKT cells as TCRβ⁺ cells that stained with a CD1d-tetramer. Indicated gates set by analysis of wildtype mice. B-E. Enumeration of NK and T cells from IFNγ-YFP reporter mice (B, C) or the mouse strains indicated (D, E). F. CD62L and CD44 expression of all IFNγ-YFP⁺CD3⁺TCRβ⁺ cells. G. Enumeration of NK cells, CD44⁺CD3⁺TCRβ⁺ T cells (CD44⁺TCRβ⁺ cells, which includes conventional T and NKT cells) and γδ T cells. In A and F the numbers represent percentage of cells in the gate shown. In B, C, G. mean ± SEM is shown. B, n ≥ 4 for all groups and pooled from 4 separate experiments. C, n ≥ 4 for all groups and pooled from 5 separate experiments. In D, E, each dot refers to one mouse. D, n ≥ 6 for all groups and pooled from 2 separate experiments. E, n ≥ 6 for all groups and pooled from 2 separate experiments. G, n ≥ 7 for all groups and pooled from ≥ 3 separate experiments **. P < 0.01, ***. P < 0.005.