Table 3. Nucleotide diversities (π) and pairwise strain genetic distances within and among the three most common species of the Colletotrichum gloeosporioides species complex causing leaf anthracnose on tea-oil trees in southern China.
| Species comparisons | Genetic distance1 | Nucleotide diversity (π)2 |
|---|---|---|
| Within C. fructicola | 0.249 ± 0.190 (N = 2080) | 0.00238 |
| Within C. siamense | 0.474 ± 0.211 (N = 253) | 0.00774 |
| Within C. gloeosporioides | 0.337 ± 0.167 (N = 28) | 0.00457 |
| Between C. fructicola and C. siamense | 1.955 ± 0.713 (N = 1495) | 0.01698 (net 0.01191) |
| Between C. siamense and C. gloeosporioides | 3.419 ± 0.1764 (N = 184) | 0.03155 (net 0.02539) |
| Between C. fructicola and C. gloeosporioides | 3.108 ± 0.132 (N = 520) | 0.02857 (net 0.02510) |
1 The genetic distances here represent the mean ± standard deviation of pairwise nucleotide differences (in percentages, % of nucleotide differences) between pairs of isolates. The percentages of pairwise nucleotide differences between isolates were calculated based on the concatenated sequences of three gene fragments (ITS, CL and GD, totaling 1362 bp). The strains included in this analysis are shown in Fig 2. While the reference strains for each of the three species shown in Fig 2 were all included but only one representative of each unique multilocus genotype for the new strains obtained in this study was included here. The number of isolates included in the calculations for the three species were 65, 8, and 23 respectively for C. fructicola, C. gloeosporioides, and C. siamense. N refers to the number of pairwise isolates used to obtain the mean and standard deviations of the genetic distances. The three between species divergences were all significantly greater than those within species divergences at P<0.001.
2 the standard nucleotide diversities within and between pairs of species. Values within parenthesis refer to net nucleotide diversity differences between pairs of species.