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. 2016 Jun 14;11(6):e0157079. doi: 10.1371/journal.pone.0157079

Fig 2. UBR5 is required for RA-mediated induction of Shh.

Fig 2

Pools of murine E14 mES cells expressing either scrambled control (SCR) or Ubr5 shRNAi were tested for (A) UBR5 expression by SDS-PAGE and Western blotting, using Tubulin as a loading control; (B) RA-mediated Shh induction by qRT-PCR (n = 3, relative expression levels to β-actin, s.e.m indicated) or (C) expression analysis of the indicated ES cell pluripotentcy markers in shRNAi pools or parental ES cells by sqRT-PCR. For (B) comparison of all matched time points post-RA-treatment between control and Ubr5 shRNAi pools revealed statistically significant differences (p = <0.01), apart from the comparison between control and Ubr5.2 at 24 hours, which was not significant (ns). Statistical analysis by one-way ANOVA and Tukey multiple comparison test.