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(A-D) qRT-PCR analysis of Prx1-Cre (Con) and Prx1-Cre;Ubr5mt*/mt* (Ubr5mt*/mt*) E13.5 or E15.5 embryonic fore- and hind-limbs for Ptch1 (A,B) and Gli1 (C,D) expression. qRT-PCR of target genes were normalised against β-actin (n = ≥3, s.e.m indicated). Statistical analysis by Students t-test. ns = not significant.
