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In situ hybridisation for expression of Hh signalling components Ptch1 (A,B) and Shh (C,D) in E11.5 embryos of Prx1-Cre (Con) and Prx1-Cre;Ubr5mt*/mt* (Ubr5mt*/mt*) embryos. Analysis revealed no significant effects on either Shh or Ptch1 expression patterns in the Ubr5mt*/mt* embryos (representative image from n = >4 of each genotype). Arrowheads indicate Shh ZPA expression domains. Scale bar = 0.5mm.
