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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Acta Biomater. 2015 Oct 24;29:42–51. doi: 10.1016/j.actbio.2015.10.039

Fig. 2.

Fig. 2

Chondrogenesis of ESC aggregates with incorporated MPs. ESC aggregates were cultured for 14 days in either basal media (BM) or differentiation media (DM). (A) Real-time PCR analysis of gene expression of chondrogenic markers. (B) SGAG content extracted from ESC aggregates was quantified by DMMB assay and normalized to DNA content. n=4, *P<0.05 vs. No MP, $P<0.05 vs. 1:3 MP under the same culture condition, #P<0.05 vs. BM. (C) sGAG deposition (red) in aggregates was visualized by safranin O and fast green counterstaining.