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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Acta Biomater. 2015 Oct 24;29:42–51. doi: 10.1016/j.actbio.2015.10.039

Fig. 3.

Fig. 3

Osteogenesis of ESC aggregates with incorporated MPs. ESC aggregates were cultured for 14 days in either basal media (BM) or differentiation media (DM). (A) Real-time PCR analysis of the gene expression of osteogenic markers. (B) Phosphate and calcium content extracted from aggregates were quantified colorimetrically and normalized to DNA content. n=4, *P<0.05 vs. No MP and $P<0.05 vs. 1:3 under the same culture; #P<0.05 vs. BM. (C) The presence of calcium and phosphate deposition within ESC aggregates was visualized by alizarin red staining (red) and von Kossa (black) / fast red counter staining, respectively.