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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Biomaterials. 2015 Oct 21;76:66–75. doi: 10.1016/j.biomaterials.2015.10.043

Figure 2.

Figure 2

GMA MP incorporation increases MMP levels in ESC aggregates. (A) Overall MMP levels, including active and total MMP activities for spheroids with and without MPs, were analyzed using a FRET-conjugated cleavable peptide-based assay. (B-C) Expression levels of pro (arrow) and active (arrowhead) forms of MMP-2 in spheroid lysates were analyzed by gel zymography (B) and densitometry (C) analysis. (D) Zymography for MMP-2 in D7 spheroids plated on either TCPS or GMA-coated TCPS. Arrow indicates MMP-2 pro form, and arrowhead indicates MMP-2 active form. * P<0.05 (n ≥ 3)