Skip to main content
. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Biomaterials. 2015 Oct 21;76:66–75. doi: 10.1016/j.biomaterials.2015.10.043

Figure 5.

Figure 5

Inhibition of MMP abrogates MP-induced changes in EMT-related and lineage-specific gene expression in day 7 ESC aggregates. The aggregates were treated with an MMP inhibitor (50 μM Ro32-3555) from day 5 of differentiation. Gene expression levels of primitive streak markers (A), EMT and early mesendodermal markers (B), and lineage specific markers (C) at day 7 of differentiation were determined by real-time PCR. Relative fold change over ESC gene expression level was calculated for all samples. * P<0.05 (n ≥ 4).