Fig. 6.

TGFβ is responsible for the microglial pro-neurogenic activity in prion disease. (A) Immunohistochemical analysis of the localisation of TGFβ (brown) in microglial cells (Iba1⁺, blue) in ME7 mice. Representative examples of double-positive cells are indicated by arrowheads. (B) TGFβ mRNA expression analysed by RT-PCR in the hippocampus of NBH+AdDL70-3, NBH+AdDcn, ME7+AdDL70-3 or ME7+AdDcn mice. Expression of mRNA represented as mean ± SEM and indicated as relative expression compared to the housekeeping gene (GAPDH) using the 2^−ΔΔCT method. (C) Analysis of the density of neural progenitor cells (NPCs; DCX⁺) at the subgranular layer (SGL) of the dentate gyrus (DG) in NBH+AdDL70-3, NBH+AdDcn, ME7+AdDL70-3 or ME7+AdDcn mice. Quantified data expressed as mean ± SEM of the number of DCX⁺ cells/mm². Scale bar in (A) 50 μm. Statistical differences for connected bars: ^*p < 0.05. Data were analysed with a two-way ANOVA showing statistical comparisons arising from a post-hoc Tukey test (n = 4–6).