(
A) Recordings from an inside-out patch containing two TRPV2 QM channels in the presence of 100 nM RTx at +90 mV. The voltage protocol used was the same as in
Figure 6A. The red continuous horizontal lines indicate the zero-current level (closed channel - C) and the dotted lines the current level for an open channel (O
1) or two simultaneously open channels (O
2). (
B) Normalized all-points histogram from all recordings obtained at +90 mV from the patch in (
A). The histogram was normalized to the total area under the curve. (
C) NP
o per sweep (where N is likely to be 2) calculated for all recordings at +90 as in (
A) from the same patch shown as a function of time. The dotted red line denotes an NP
o of 0. (
D) Single-channel TRPV2 QM recordings from an inside-out patch at +90 mV in the presence of 400 nM RTx. The red continuous horizontal lines indicate the zero-current level (closed channel - C) and the dotted lines the current level for an open channel (
O). (
E) Normalized all points-histogram from recordings at +90 mV and 400 nM RTx from the patch in (
D). (
F) P
o per sweep as a function of time calculated for the patch in (
D) at +90 mV and 400 nM RTx. The dotted red line denotes a P
o of 0.