Plants were grown under standard greenhouse conditions and samples were taken from young (y), expanding (i), and fully expanded (m) leaves at three developmental stages (1–3; cf.
Figure 3—figure supplement 1). Metabolite levels were determined by UPLC analysis. The values represent the peak height for each compound divided by 10.000 and normalized to the fresh weight (FW), resulting in the normalized response/FW. Error bars represent the SD (n = 3 plants per line). Different letters above the bars indicate significant differences as determined by One-way ANOVA (p<0.05) and the Holm-Sidak post-test.