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. 2016 Jun 9;11:2721–2736. doi: 10.2147/IJN.S102450

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© 2016 Zheng et al. This work is published and licensed by Dove Medical Press Limited

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The apoptosis of C6 glioma cells was determined by FCM using Annexin V-FITC/PI staining.

Notes: Normal saline (A), empty micelles (B), free curcumin (C–E), and Cur/MPEG-PLA micelles (F–H) were added to wells at different concentrations, respectively (Cur: 3.125 μg/mL for C and F, 6.25 μg/mL for D and G, and 12.5 μg/mL for E and H). After incubation for 24 hours, cells were harvested, stained with Annexin V-FITC/PI, and analyzed with FCM.

Abbreviations: FCM, flow cytometry; FITC, fluorescein isothiocyanate; PI, propidium iodide; Cur, curcumin; MPEG-PLA, monomethoxy poly(ethylene glycol)-poly(lactide) copolymer.