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CAM assay.
Notes: (A) Position of the silicon O-rings in the developing blood vessels plexus of the yolk sac membrane. (B) RGB image of the O-ring interior used for counting the number of veins. (C) Green channel of the same image. (D) Binary of the segmented image used to calculate the number of branches.
Abbreviations: CAM, chorioallantoic membrane; RGB, red, green, and blue.
